Laboratories : IBS and LPCV - 01/01/2021 – 31/12/2023

Thesis project holders : David COBESSI and Robert BLANVILLAIN
Young thesis researcher : Soumiya SANKARI MUTHUKUMAR

Abstract: The thesis investigated activity of the superoxide dismutases PAP4 and PAP9 from the PEP. The PEP envelope was calculated at 27,5 Å resolution, and the CSP41b 3D structure solved at 3.4 Å resolution. Its interaction with PRIN2 was investigated using biophysical experiments and tested in onion epidermal cells using bimolecular fluorescence complementation assay. For fishing PRIN2 interactors, a proximity labelling strategy was designed. Genetic constructions were cloned and tested in transient experiments, proving its feasibility. These studies are part of a broader project that aims at highlighting functional innovations in angiosperms around PEP-specific plastid transcription.




Publications :
Ruedas, R., Muthukumar, S.S., Kieffer-Jaquinod, S. et al. (2022). Three-dimensional envelope and subunit interactions of the plastid-encoded RNA polymerase from Sinapis alba. Int. J. Mol. Sci., 23, 9922.
Favier, A., Gans, P., Boeri Erba, E. et al. (2021). The plastid-encoded RNA polymerase-associated protein PAP9 is a superoxide dismutase with unusual structural features. Front. Plant Sci., 12, 668897.