Laboratories : DCM and DPM - 01/10/2020 – 31/12/2023

Thesis project holders : Michael HOLZINGER and Corinne RAVELET
Young thesis researcher : Essohanam BEKE

Abstract: The detection of the hormone arginine vasopressin (AVP), an important biomarker for a series of diseases, needs currently expensive and time-consuming techniques. This project aimed to develop an electrochemical setup based on impedimetric transduction (EIS) using a specific aptamer (anti-AVP). One challenge was to determine the optimal immobilization strategy for reliable quantification of this hormone. Another was to decrease the limit of detection (LOD) down to the picomolar range. These targets could be implemented by establishing strict protocols for the formation of the sensing device.

The obtained results : A LOD in the sub-picomolar range was obtained with a linear range of four orders of magnitude.